Measurement Of Alkaline Phosphatase Activity And 4-Nitrophenol Production In Serum

The Beer-Lambert LawAlkaline phosphatase is a very widely distributed enzyme.  As the name implies it catalyses (speeds up) the hydrolysis of phosphate esters (e.g. glucose phosphate, ATP etc.) under alkaline conditions, i.e. at pH values above 7.  Although the physiological function of the enzyme is not properly understood, the assay of the enzyme in samples of serum is very commonly performed in biochemistry laboratories in ho…